Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia
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Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia
In 2017, a variety of orange-colored petunias in the market turned out to be genetically modified (GM) without official authorization for commercialization. sequence analysis suggested these plants declared most probably derived from the plant transformation experiments done in the 1980s. For a deeper understanding of how GM petunias entry classical breeding programs around the world, and whether they came from a single source or not, we do molecular genetic characterization of T-DNA integration sites in a variety of GM petunia cultivars and breeding lines.
By way of running the genome, we isolated a T-DNA sequence is different, which is located at the junction between the T-DNA (s) and petunia DNA. Based on the results we conclude that there are at least two copies of the T-DNA of different lengths. This is supported by Southern blot analysis. For T-DNA1, 3′-junction sequence is isolated, while the 5′-junction is still unclear.
Conversely, for T-DNA2, the sequence 5′-junction isolated, while the sequence is isolated from the 3′-region consists only of the T-DNA, but does not include the intersection of the T-DNA into the DNA petunias. We developed a PCR primer for a specific event and screened a set of three orange petunia cultivars GM and GM 126 offspring from a commercial breeding program. We show that the second copy of the T-DNA that is present in all tested samples of petunias our GM, which supports the assumption of a single transgenic origin of petunias GM declared. Most likely, two T-DNA integrated into the genome petunia close.
Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia
Case report Polymerase Chain Reaction-Confirmed COVID-19 in Patients With Right Ventricular Thrombus and Bilateral Deep Vein Thrombosis
Coronavirus new, two acute respiratory syndrome coronavirus (SARS-CoV-2), which causes a highly contagious coronavirus 2019 (COVID-19) has caused a global health crisis unprecedented. infected patients have been shown to trigger a high inflammatory response, increasing the risk of thrombosis. We report a case of polymerase chain reaction (PCR) -confirmed COVID 19 in the male Hispanics who do not have a past medical history who presented to the ED with upper respiratory tract symptoms including shortness of breath and cough, requiring continuous positive airway pressure (CPAP) therapy
He was found to have right ventricular thrombus (RVT) and bilateral deep vein thrombosis (DVT) on the day of admission, which was detected on transthoracic echocardiogram and venous duplex ultrasound, respectively. Patients started on enoxaparin sodium therapy, leading to improved oxygenation, and he was eventually relegated to the medical floor for further management.
In this study, SYBR Green-based real-time quantitative polymerase chain reaction (qPCR) assay was developed for the rapid detection of swine parvovirus (PPV) 6. Primer pairs targeting conserved regions PPV6 capsid gene was designed. sensitivity analysis revealed lower limit of detection of real-time PCR based on SYBR Green to 47.8 copies / uL, indicating it is 1000 times higher than that found in conventional PCR probe.
This test is specific and does not show cross-species amplification with six other swine viruses. Test shows high repeatability and reproducibility; coefficients intra and inter-assay variation were 0.79% and 0.42%, respectively.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Apolipoprotein B (APOB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Apolipoprotein B (APOB) in samples from serum, plasma or other biological fluids.
Description: Quantitative competitive ELISA kit for measuring Pig Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Pig Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Apolipoprotein B (APOB) in samples from Serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitative competitive ELISA kit for measuring Duck Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Duck Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Fish Apolipoprotein B (APOB) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Fish Apolipoprotein B (APOB) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Goat Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Goat Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Apolipoprotein B (APOB) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Apolipoprotein B (APOB) in samples from serum, plasma or other biological fluids.
Description: Quantitative competitive ELISA kit for measuring Horse Apolipoprotein B (APOB) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Horse Apolipoprotein B (APOB) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Mouse Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Mouse Apolipoprotein B(APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Sheep Apolipoprotein B (APOB) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Sheep Apolipoprotein B (APOB) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Apolipoprotein B (APOB) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitative competitive ELISA kit for measuring Bovine Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Bovine Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Chicken Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Chicken Apolipoprotein B(APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Hamster apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Hamster apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative competitive ELISA kit for measuring Guinea pig Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Guinea pig Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Enzyme-linked immunosorbent assay kit for quantification of Duck Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Goat Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Horse Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Bovine Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Chicken Apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Hamster apolipoprotein B (APOB) in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A sandwich ELISA kit for detection of Apolipoprotein B from Pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Apolipoprotein B from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Wide-range Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Wide-range Mouse Apolipoprotein B (APOB) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Wide-range Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Wide-range Mouse Apolipoprotein B (APOB) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Wide-range Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Wide-range Mouse Apolipoprotein B (APOB) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Wide-range Mouse Apolipoprotein B (APOB) in serum, plasma and other biological fluids.
Wide-range Mouse Apolipoprotein B (APOB) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Wide-range Mouse Apolipoprotein B (APOB) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Apolipoprotein B from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA kit for detection of Apolipoprotein B from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
High Sensitive Human Apolipoprotein B (APOB) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
High Sensitive Human Apolipoprotein B (APOB) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Apolipoprotein B (APOB) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
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Positive detection rate of 180 clinical samples with SYBR Green-based real-time PCR and conventional PCR was 12.22% (22/180) and 4.44% (8/180), respectively. Our method is sensitive, specific, and reproducible. The use of SYBR Green-based real-time PCR detection may be suitable for clinical and epidemiological investigation of PPV6.
