Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

Background: Non-responding pneumonia is responsible for the largest mortality of community-acquired pneumonia (CAP). However, until now, it is not clear whether viral infection plays an important role in the etiology of the non-corresponding cap and if there is a significant difference in the clinical characteristics between the viral non-respecting cap. respective.

Methods: From 2016 to 2019, patients with non-responding capsules have been recorded retrospectively in our study. All patients received bronchoalvolar washing (ball) and virus detection in the ball fluid by reaction of the polymerase multiplex chain (PCR) and clinical data, laboratories and radiographic laboratories were collected.

Results: A total of 43 patients were included. The median age was 62 and 65.1% of patients were men. Overall, 20 patients (46.5%) have been identified with viral infection. Of these viruses, the influenza virus (n = 8) and adenovirus (n = 7) have been detected more often, and other people included the herpes simplex virus, human enterovirus, cytomegalovirus The human coronavirus 229E, rhinovirus and the parainfluenza virus. Compared to the non-respective non-respective cap, only a soil opacity combined with the consolidation was a more common imaging manifestation in the non-corresponding viral cap. However, no obvious difference was found in the clinical and laboratory conclusions between the presence and absence of viral infections.

Conclusions: Viral infections were particularly common in adults with a non-corresponding cap. The soil opacity associated with the consolidation was a specific imaging manifestation for the non-corresponding viral cap, while clinical and laboratory data showed no obvious difference between the non-respective viral and non-respective cap.

The reaction of the quantitative polymerase chain of methylation-sensitive methylation allows rapid, precise and precise detection of the methylation state of the T cell area of ​​the T cell (Treg) – specific demethylation in primary human rules

Human regulatory T cells (Tregs) have been involved in cancer immunotherapy and are also emerging cellular therapy for the treatment of several indications. Although Trega stability during ex vivo culture has improved, methods for evaluating Treg stability, such as bisulfite sequencing Sanger to determine the state of methylation of the demethylated region specific to the Treg (TSDR) remained unchanged. Bisulfite Sanger Sequencing is not only expensive and cumbersome to perform, it is incorrect because of relatively low read accounts. The sequencing of the next generation bisulfite, although more precise, is a less accessible method.

In this study, we describe the application of restriction enzymes sensitive to methylation (MSRE) and Quantitative PCR (QPCR) to determine the methylation status of the TSDR. Using known Tregs and Non-Tregs reports, we show that Msre-QPCR can distinguish the methylation status of the TSDR in cell populations containing a growing proportion of Tregs from 0 to 100%. In a comparison with values ​​obtained from a bisulfite sequencing approach established to determine the methylation status of the TSDR, our MSRE-QPCR results were 5% on average for all samples with a high percentage (> 70% ) from Tregs, reinforcing that MSRE-QPCR can be completed in less time than other methods with the same level of precision.

The value of this assay was then demonstrated by quantifying the differences in treated Treg methylation status with and without rapamycin during an ex vivo expansion culture. Together, we show that our new msre-QPCR application to the TSDR is an optimal test for a precise assessment of TREG purity.

Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia
Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

Analysis of extracellular bladder vesicle proteins using a digital polymerase chain reaction amplification

It is necessary for new analytical techniques to study the composition of simple extracellular vesicles (EV). These techniques are necessary to improve the understanding of the populations of EV heterogeneous in order to allow the identification of unique subpopulations and to allow detection of more than earlier and sensitive diseases. Due to the small size of EV and their low protein content, ultrachage sensitivity technologies are needed.

Here, a method of amplifying the reaction of the digital polymerase chain (IDDPCR) is described to allow multiplexed single-EV protein profiling. Antibody DNA conjugates are used to label EV, followed by a stochastic microfluidic incorporation of the single EV in the droplets. In situ PCR with fluorescent journalist probes converts and amplifies the barcode signal for subsequent reading by Goutteletelterie imaging. In these principle studies, it is shown that the analysis of the multiplex protein is possible in a single EV, opening the door for future analyzes. In this study, a poly-L-lysine magnetic pearl technique (PLL) of vancomycin (PLL) is developed for an insulation of gram-positive bacteria. The method combines a magnetic separation with a PCR (MPCR) multiplex dosage and gel electrophoresis for easy and fast detection of Bacillus cereus.

Anti-Taq DNA Polymerase Antibody

E8EM1701-49 100ul
EUR 275
Description: Available in various conjugation types.

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 606.3
Description: Antibody raised against DNA polymerase beta

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Anti-Taq DNA Polymerase Antibody [3C3]

EM1701-49 100ul
EUR 210
Description: Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

Anti-DNA Polymerase Antibody

E38A6546 100ug/100ul
EUR 225
Description: Available in various conjugation types.

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

DF6552 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase beta Antibody

AF0148 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase iota Antibody

E38PA5564 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase theta Antibody

DF13563 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase alpha Antibody

AF5235 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Polymerase alpha Antibody

DF3082 100ul
EUR 280
Description: Human

DNA Polymerase alpha Antibody

AF5235-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

AF5235-200ul 200ul
EUR 350

DNA Polymerase theta Antibody

DF13563-100ul 100ul
EUR 280

DNA Polymerase theta Antibody

DF13563-200ul 200ul
EUR 350

DNA Polymerase alpha Antibody

DF3082-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

DF3082-200ul 200ul
EUR 350

Anti-POLK/Dna Polymerase Kappa Antibody

A01987 100ug
EUR 518.4
Description: Goat Polyclonal POLK/Dna Polymerase Kappa Antibody. Validated in WB and tested in Human.

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

DNA Polymerase Mu (POLM) Antibody

abx236614-100ug 100 ug
EUR 661.2

DNA Polymerase Mu (POLM) Antibody

20-abx217851
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Mu (POLM) Antibody

20-abx141146
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Nu (POLN) Antibody

abx028202-400ul 400 ul
EUR 627.6

DNA Polymerase Nu (POLN) Antibody

abx028202-80l 80 µl
EUR 343.2

DNA Polymerase Mu (POLM) Antibody

20-abx323923
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Eta (POLH) Antibody

abx117166-100ug 100 ug
EUR 560.4

DNA Polymerase Eta (POLH) Antibody

abx036483-100ug 100 ug
EUR 469.2

DNA Polymerase Eta (POLH) Antibody

20-abx001515
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Eta (POLH) Antibody

20-abx302606
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA polymerase eta Antibody / POLH

R32359 100 ug
EUR 356.15
Description: DNA polymerase eta (POLH), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

DNA Polymerase delta 3 antibody

70R-50825 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 3 antibody

DNA Polymerase alpha 1 antibody

70R-50225 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase alpha 1 antibody

DNA Polymerase delta 1 antibody

70R-50227 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase delta 1 antibody

DNA Polymerase delta 3 Antibody

20-abx008311
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase delta 1 Antibody

20-abx008851
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase alpha 1 Antibody

20-abx008853
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase delta 3 Antibody

E38PA2506 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase gamma 2 Antibody

E38PA4161 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase alpha 1 Antibody

E38PA1906 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase delta 1 Antibody

E38PA1908 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase Iota (POLI) Antibody

abx236612-100ug 100 ug
EUR 577.2

DNA Polymerase Iota (POLI) Antibody

20-abx333843
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

abx036752-100ug 100 ug
EUR 469.2

DNA Polymerase Iota (POLI) Antibody

abx028115-400ul 400 ul
EUR 627.6

DNA Polymerase Iota (POLI) Antibody

abx028115-80l 80 µl
EUR 343.2

DNA Polymerase Iota (POLI) Antibody

20-abx323859
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Iota (POLI) Antibody

20-abx001585
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase iota Antibody / POLI

RQ4298 100 ug
EUR 356.15
Description: DNA polymerase iota is an enzyme that in humans is encoded by the POLI gene. The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.

DNA Polymerase beta Antibody / POLB

RQ5162 100ul
EUR 356.15
Description: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. [RefSeq]

DNA polymerase beta Antibody / POLB

R31898 100 ug
EUR 356.15
Description: Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

DNA polymerase delta cat antibody

70R-12690 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase delta cat antibody

DNA polymerase delta p50 Antibody

DF13788 100ul
EUR 280
Description: Human,Mouse,Rat

DNA polymerase delta p50 Antibody

DF13788-100ul 100ul
EUR 280

DNA polymerase delta p50 Antibody

DF13788-200ul 200ul
EUR 350

DNA Polymerase Kappa (POLK) Antibody

abx236613-100ug 100 ug
EUR 610.8

DNA Polymerase Kappa (POLK) Antibody

abx031322-400ul 400 ul
EUR 627.6

DNA Polymerase Kappa (POLK) Antibody

abx031322-80l 80 µl
EUR 343.2

DNA Polymerase Sigma (POLS) Antibody

abx029151-400ul 400 ul
EUR 627.6

DNA Polymerase Sigma (POLS) Antibody

abx029151-80l 80 µl
EUR 343.2

DNA Polymerase Kappa (POLK) Antibody

20-abx126383
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126384
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Kappa (POLK) Antibody

20-abx126385
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Theta (POLQ) Antibody

20-abx322965
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

DNA Polymerase Kappa (POLK) Antibody

20-abx338646
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Polymerase Theta (POLQ) Antibody

abx432614-200ul 200 ul
EUR 343.2

DNA Polymerase Kappa (POLK) Antibody

20-abx004686
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

DNA Polymerase Theta (POLQ) Antibody

20-abx013320
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 571.2

DNA Polymerase Zeta (REV3L) Antibody

20-abx133309
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase epsilon 2 antibody

70R-50228 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase epsilon 2 antibody

Human DNA Polymerase beta Antibody

33292-05111 150 ug
EUR 313.2

DNA Polymerase epsilon 2 Antibody

20-abx008850
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase Zeta (REV3L) Antibody

20-abx013311
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase epsilon 2 Antibody

E38PA1909 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase Lambda (POLL) Antibody

20-abx217850
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

DNA Polymerase Lambda (POLL) Antibody

abx332616-100ul 100 ul
EUR 510

DNA Polymerase Lambda (POLL) Antibody

20-abx013831
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase Lambda (POLL) Antibody

abx034579-400ul 400 ul
EUR 627.6

DNA Polymerase Lambda (POLL) Antibody

abx034579-80l 80 µl
EUR 343.2

DNA Polymerase Lambda (POLL) Antibody

20-abx328341
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Vancomycin has been used as a molecular ligand between the MB and the d-alanyl-d-alanine boundaries on the surface of the cell wall of B. Cereus. The PLL served as a flexible molecular attachment between the MB and the van that reduced the obstacle to maintaining the biological activity of the van. The MB-PLL-VAN Capture nanoprobes have presented excellent capture and insulation efficiency for B. Cereus in milk matrix samples with interference from the complex food matrix. The following MPCR test showed a high specificity for the 4 target genes of B. Cereus, ENTFM, CESB, CER and 16S RNRA genes used to obtain effective genotyping and detection.