Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

Background: Non-responding pneumonia is responsible for the largest mortality of community-acquired pneumonia (CAP). However, until now, it is not clear whether viral infection plays an important role in the etiology of the non-corresponding cap and if there is a significant difference in the clinical characteristics between the viral non-respecting cap. respective.

Methods: From 2016 to 2019, patients with non-responding capsules have been recorded retrospectively in our study. All patients received bronchoalvolar washing (ball) and virus detection in the ball fluid by reaction of the polymerase multiplex chain (PCR) and clinical data, laboratories and radiographic laboratories were collected.

Results: A total of 43 patients were included. The median age was 62 and 65.1% of patients were men. Overall, 20 patients (46.5%) have been identified with viral infection. Of these viruses, the influenza virus (n = 8) and adenovirus (n = 7) have been detected more often, and other people included the herpes simplex virus, human enterovirus, cytomegalovirus The human coronavirus 229E, rhinovirus and the parainfluenza virus. Compared to the non-respective non-respective cap, only a soil opacity combined with the consolidation was a more common imaging manifestation in the non-corresponding viral cap. However, no obvious difference was found in the clinical and laboratory conclusions between the presence and absence of viral infections.

Conclusions: Viral infections were particularly common in adults with a non-corresponding cap. The soil opacity associated with the consolidation was a specific imaging manifestation for the non-corresponding viral cap, while clinical and laboratory data showed no obvious difference between the non-respective viral and non-respective cap.

The reaction of the quantitative polymerase chain of methylation-sensitive methylation allows rapid, precise and precise detection of the methylation state of the T cell area of ​​the T cell (Treg) – specific demethylation in primary human rules

Human regulatory T cells (Tregs) have been involved in cancer immunotherapy and are also emerging cellular therapy for the treatment of several indications. Although Trega stability during ex vivo culture has improved, methods for evaluating Treg stability, such as bisulfite sequencing Sanger to determine the state of methylation of the demethylated region specific to the Treg (TSDR) remained unchanged. Bisulfite Sanger Sequencing is not only expensive and cumbersome to perform, it is incorrect because of relatively low read accounts. The sequencing of the next generation bisulfite, although more precise, is a less accessible method.

In this study, we describe the application of restriction enzymes sensitive to methylation (MSRE) and Quantitative PCR (QPCR) to determine the methylation status of the TSDR. Using known Tregs and Non-Tregs reports, we show that Msre-QPCR can distinguish the methylation status of the TSDR in cell populations containing a growing proportion of Tregs from 0 to 100%. In a comparison with values ​​obtained from a bisulfite sequencing approach established to determine the methylation status of the TSDR, our MSRE-QPCR results were 5% on average for all samples with a high percentage (> 70% ) from Tregs, reinforcing that MSRE-QPCR can be completed in less time than other methods with the same level of precision.

The value of this assay was then demonstrated by quantifying the differences in treated Treg methylation status with and without rapamycin during an ex vivo expansion culture. Together, we show that our new msre-QPCR application to the TSDR is an optimal test for a precise assessment of TREG purity.

Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia
Detection of Viruses by Multiplex Real-Time Polymerase Chain Reaction in Bronchoalveolar Lavage Fluid of Patients with Nonresponding Community-Acquired Pneumonia

Analysis of extracellular bladder vesicle proteins using a digital polymerase chain reaction amplification

It is necessary for new analytical techniques to study the composition of simple extracellular vesicles (EV). These techniques are necessary to improve the understanding of the populations of EV heterogeneous in order to allow the identification of unique subpopulations and to allow detection of more than earlier and sensitive diseases. Due to the small size of EV and their low protein content, ultrachage sensitivity technologies are needed.

Here, a method of amplifying the reaction of the digital polymerase chain (IDDPCR) is described to allow multiplexed single-EV protein profiling. Antibody DNA conjugates are used to label EV, followed by a stochastic microfluidic incorporation of the single EV in the droplets. In situ PCR with fluorescent journalist probes converts and amplifies the barcode signal for subsequent reading by Goutteletelterie imaging. In these principle studies, it is shown that the analysis of the multiplex protein is possible in a single EV, opening the door for future analyzes. In this study, a poly-L-lysine magnetic pearl technique (PLL) of vancomycin (PLL) is developed for an insulation of gram-positive bacteria. The method combines a magnetic separation with a PCR (MPCR) multiplex dosage and gel electrophoresis for easy and fast detection of Bacillus cereus.

Anti-DNA polymerase eta Antibody

STJ502747 100 µg
EUR 571.2

Anti-DNA polymerase theta antibody

STJ71548 100 µg
EUR 312

anti-DNA Polymerase epsilon

YF-PA19269 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19270 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19271 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA Polymerase epsilon

YF-PA19272 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA Polymerase epsilon

anti-DNA polymerase alpha

YF-PA24412 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase alpha

anti-DNA polymerase beta

YF-PA24413 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA Polymerase epsilon

YF-PA26240 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase epsilon

anti-DNA polymerase beta

YF-PA13864 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase beta

anti-DNA polymerase beta

YF-PA13865 100 ul
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase beta

anti-DNA polymerase eta

YF-PA13871 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase eta

anti-DNA polymerase eta

YF-PA13872 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase eta

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

anti-DNA polymerase? antibody (rabbit antiserum)

70-041 50uL
EUR 496.8
Description: The anti-DNA polymerase? antibody (rabbit antiserum) is available in Europe and for worldwide shipping via Gentaur.

Anti-POLK/Dna Polymerase Kappa Antibody

A01987 100ug
EUR 518.4
Description: Goat Polyclonal POLK/Dna Polymerase Kappa Antibody. Validated in WB and tested in Human.

Anti-DNA Polymerase lambda/POLL Antibody

A00959-2 100ug/vial
EUR 352.8

Anti-DNA Polymerase iota/POLI Antibody

A01376-1 100ug/vial
EUR 352.8

Anti-DNA Polymerase beta Monoclonal Antibody

M01946 100ug
EUR 476.4
Description: Rabbit Monoclonal DNA Polymerase beta Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat.

Anti-DNA Polymerase beta/POLB Antibody

PB9812 100ug/vial
EUR 352.8

Anti-DNA polymerase eta/POLH Antibody

PB9813 100ug/vial
EUR 352.8

Anti-DNA polymerase eta Antibody BIOTIN

STJ502748 100 µg
EUR 703.2

Anti-DNA polymerase eta Antibody FITC

STJ502749 100 µg
EUR 703.2

Anti-DNA Polymerase theta (1C11)

YF-MA17459 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase theta

anti-DNA Polymerase Kappa/POLK

YF-PA19042 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19043 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase Kappa/POLK

YF-PA19044 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA Polymerase Kappa/POLK

anti-DNA Polymerase epsilon p59

YF-PA24414 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase epsilon p59

anti-DNA Polymerase Kappa/POLK

YF-PA26181 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Polymerase Kappa/POLK

anti-DNA polymerase delta p50

YF-PA13866 50 ug
EUR 435.6
Description: Mouse polyclonal to DNA polymerase delta p50

anti-DNA polymerase delta p50

YF-PA13867 100 ug
EUR 483.6
Description: Rabbit polyclonal to DNA polymerase delta p50

Anti-DNA polymerase alpha (3C11)

YF-MA14797 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA polymerase alpha

Anti-DNA Polymerase iota (8G9)

YF-MA11349 100 ug
EUR 435.6
Description: Mouse monoclonal to DNA Polymerase iota

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase lambda antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase beta Antibody

20-abx013060
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase alpha Antibody

20-abx013375
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

DNA Polymerase beta Antibody

20-abx008852
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10202 1000U
EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500
EUR 614.4

Taq DNA Polymerase

9001-500
EUR 229.2

PFU DNA Polymerase

9003-2500
EUR 614.4

PFU DNA Polymerase

9003-500
EUR 216

Laq? DNA Polymerase

9004-2500
EUR 732

Laq? DNA Polymerase

9004-500
EUR 261.6

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA Polymerase

BA00205 2500U
EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00303 500U
EUR 147.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00304 1000U
EUR 235.2
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

RedTaq DNA Polymerase

BA00305 2500U
EUR 495.6
Description: High quality RedTaq polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00503 500U
EUR 174
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00504 1000U
EUR 286.8
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

Pfu DNA Polymerase

BA00505 2500U
EUR 626.4
Description: High quality Pfu polymerase for different PCR variations and downstream applications.

BIOTAQ DNA Polymerase

BIO-21040 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21046 250 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21047 500 Units Ask for price

IMMOLASE DNA Polymerase

BIO-21048 5000 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21051 250 Units Ask for price

ACCUZYME DNA Polymerase

BIO-21052 500 Units Ask for price

BIOTAQ DNA Polymerase

BIO-21060 2500 Units Ask for price

MangoTaq DNA Polymerase

BIO-21078 5000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21082 2000 Units Ask for price

MangoTaq DNA Polymerase

BIO-21083 1000 Units Ask for price

VELOCITY DNA Polymerase

BIO-21098 250 Units Ask for price

VELOCITY DNA Polymerase

BIO-21099 500 Units Ask for price

MyTaq DNA Polymerase

BIO-21105 500 units Ask for price

MyTaq DNA Polymerase

BIO-21105/S Sample Ask for price

MyTaq DNA Polymerase

BIO-21106 2500 units Ask for price

MyTaq DNA Polymerase

BIO-21107 5000 units Ask for price

MyFi DNA Polymerase

BIO-21117 250 Units Ask for price

MyFi DNA Polymerase

BIO-21117/S Sample Ask for price

MyFi DNA Polymerase

BIO-21118 500 Units Ask for price

MyFi DNA Polymerase

BIO-21119 2500 Units Ask for price

Ranger DNA Polymerase

BIO-21121 250 Units Ask for price

Ranger DNA Polymerase

BIO-21121/S Sample Ask for price

Ranger DNA Polymerase

BIO-21122 500 Units Ask for price

Ready? DNA Polymerase

M1146-1000
EUR 398.4

Ready? DNA Polymerase

M1146-10000
EUR 1279.2

Ready? DNA Polymerase

M1146-5000
EUR 960

Breeze? DNA Polymerase

M1148-1000
EUR 1246.8

Breeze? DNA Polymerase

M1148-250
EUR 464.4

Distant? DNA Polymerase

M1150-1000
EUR 823.2

Distant? DNA Polymerase

M1150-250
EUR 333.6

Vancomycin has been used as a molecular ligand between the MB and the d-alanyl-d-alanine boundaries on the surface of the cell wall of B. Cereus. The PLL served as a flexible molecular attachment between the MB and the van that reduced the obstacle to maintaining the biological activity of the van. The MB-PLL-VAN Capture nanoprobes have presented excellent capture and insulation efficiency for B. Cereus in milk matrix samples with interference from the complex food matrix. The following MPCR test showed a high specificity for the 4 target genes of B. Cereus, ENTFM, CESB, CER and 16S RNRA genes used to obtain effective genotyping and detection.