Comparison of Periodontopathic Bacterial Profiles of Different Periodontal Disease Severity Using Multiplex Real-Time Polymerase Chain Reaction
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Comparison of Periodontopathic Bacterial Profiles of Different Periodontal Disease Severity Using Multiplex Real-Time Polymerase Chain Reaction
The parotopathic bacteria are known to have a crucial role in the pathogenesis of periodontitis. The objective of the study was to quantitatively compare the bacterial profile of patients with a different gravity of periodontal disease using mouthwash samples and sub-range zone. Further analysis was performed to evaluate the correlation between the mouth bath and two sampling methods underwritten: Paperpoint and gingival retraction cord; 114 subjects enrolled in the study and have been divided equally into three groups depending on the severity of diseases. Mouth sampling and sub-range samples were performed and the samples were quantitatively analyzed for 11 target parodontopathy bacteria using real-time Multiplex PCR.
There were statistically significant differences in bacterial accounts and the prevalence of several species between the study groups. Sampling of the mouthwash showed significant correlations with two different sub-range sampling methods with respect to the detection of several bacteria (for example, ρ = 0.793 for gingivalalis porphyromonas in severe periodontitis), involving that mouth mouth sampling can reflect the sub-window microbiota. However, the correlation was more important because the severity of the disease increased. Although bacteria in the mouth bath can become a biomarker, it may be more appropriate for the diagnosis of severe periodontitis rather than early diagnosis. Additional research is needed for the discovery of biomarkers for early diagnosis of periodontitis.
Here, we note the case of a patient with BNS receiving IBRutinib from which we have detected the early relapse by monitoring the molecular residual disease (MRD) on the basis of the MYD88 L265P mutation in the cephalo-rachidian fluid ( CSF) on the determination of the reaction of the Dropoulet digital polymerase chain. The persistent MRD increased 2 weeks before the appearance of the symptoms of relapse without any conclusion of abnormal imaging or clonal LPC evidence on the CSF cytology, flow cytometry analysis or immunofacious electrophoresis. Our results suggest that an increase in MRD levels is correlated with relapse in patients with SNBs.
Febrile and respiratory viruses determined by the reaction test of the multiplex polymerase chain and clinical diagnosis
Fecking seizure (FS) is a common benign crisis disorder for young children. Although the upper respiratory tract infection is the cause of the fever in most episodes of FS, studies to identify respiratory viruses using a multiplex polymerase chain reaction test (MPCR). rarely been made for children with FS. Medical records of children with FS between January 2015 and December 2019 have been reviewed retrospectively. Respiratory viruses identified by a fast influenza detection test and a MPCR test have been examined and seasonal distribution and the association between viral identification and input characteristics have been determined. A total of 607 Episodes of FS were analyzed: 81.1% of cases were generally generalized onic-clonic crises, 81.5% took place within 24 hours after the appearance of fever and 87.3 % continued during ≤ 5 min.
The FS complex occurred in 17.5% of the FS episodes and epilepsy was diagnosed in 2.5% of the cases followed. On the 138 MPCR tests carried out in 235 hospitalized episodes of FS, 112 (81.2%) positive for respiratory viruses: rhinovirus, enterovirus, adenovirus and the flu virus were the most frequently identified . The identified respiratory viruses showed similar seasonal distributions as observed in the infection of the respiratory tract acquired by the Community. The identification of a specific respiratory virus has not been significantly associated with the input characteristics or the development of complex FS. In conclusion, respiratory viruses, showing similar seasonal distributions with community-based respiratory tract infections and no significant association with the severity and results of FS, should not be rigorously tested in children with FS.
Comparison of Periodontopathic Bacterial Profiles of Different Periodontal Disease Severity Using Multiplex Real-Time Polymerase Chain Reaction
Utility of the reaction of the chain of the nested polymerase for the fungus in the detection of female presumed patients of invasive fungal infections and its clinical correlation and comparison with fungal culture
Objectives and objectives: The objective and purpose of this study is to detect invasive fungal infections (IFI) early and with more sensitivity by the reaction of the nested polymerase chain (PCR) for mushroom relative to culture Fungy of the folk female clinically and also explore its correlation with respect to age, the duration of symptoms, the immunocompromised state and other risk factors predisposing the IFI.
Materials and methods: In this cross-sectional study, 50 presumed patients admitted to a medical health care unit / intensive care unit (ACU / ICU) of Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, India, included ‘study. All cases have been selected on the basis of predefined inclusion and exclusion criteria. A detailed history, a clinical examination and all required surveys were carried out on all suspected patients. Blood samples were taken for the nested PCR for fungus and culture. The nested PCR was carried out on DNA samples from the DNA collected from all participants under the study.
Results: Our study comprising 50 IFIS immunocompromised suspected patients. Among the participants of the study, the most common risk factor was sweet diabetes (28%). Nearly two-thirds (60%) of cases were 50 years of age or older. About 70% of cases had a history of disease more than 2 weeks. Nested PCR for fungus is released to be positive in 21/50 patients (42%); However, fungal culture was positive in any. Among the patients admitted to ACU / ICU, 75% were neutropenic.
POLR2B, ID (POLR2B, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140kD polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (FITC)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (FITC)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (HRP)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (HRP)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (PE)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (PE)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (Biotin)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (Biotin)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) APC
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) APC
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (AP)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (AP)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 490)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 490)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 405)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 405)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 750)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 750)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 650)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 650)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 550)
POLR2A (DNA-directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-directed RNA Polymerase II Subunit A, DNA-directed RNA Polymerase III Largest Subunit, RNA-directed RNA Polymerase II Subunit RPB1, POLR2, MGC75453) (MaxLight 550)
Description: RNA polymerase II-IN-1 (compound 19iv) is a amatoxin, inhibiting RNA polymerase II (Pol II) with an IC50 value of 36.66 nM. RNA polymerase II-IN-1 has higher cytotoxicity against cancer cells and less toxic in normal cells than α-Amanitin[1].
Description: RNA polymerase II-IN-2 (compound 20iii) is a potent RNA polymerase II (Pol II) inhibitor with Ki value of 9.5 nM. RNA polymerase II-IN-2 has cytotoxicity against cancer cells, and exhibits 2 and 5 fold toxicity than α-amanitin against CHO and HEK293[1].
POLR2D, ID (POLR2D, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16kD subunit)
Conclusions: IFIs are more frequent in immunized individuals, patients with comorbidities, a long history of symptoms and an elderly population. Nested PCR for mushroom has a high sensitivity (compared to fungal culture), and they are also quick to give the results. Thus, the nested PCR for fungus can be used profitably for the early and reliable diagnosis of clinically suspected IFIs.
