Successful containment of horizontal enterovirus infection in a neonatal unit in Singapore through diagnosis by polymerase chain reaction (PCR) and direct sequence analysis

Background: Enterovirus’ epidemics (EV) often coincide with seasonal peaks in the community. However, they can also occur sporadically in neonatal units. The identification of EV infection in newborns can be difficult because they tend to have light or non-specific symptoms. This study reports an EV epidemic in the neonatal unit of KK Women’s Hospital, Singapore.

Methods: This is a simple and retrospective retrospective cohort study of newborns that had positive results for EV during the epidemic. Demographic characteristics, clinical presentations and results have been analyzed. The control measures used to limit the propagation of the infection are reported.

Results: A total of 7 cases of EV infection have been identified. Their median weighing on birth and their gestational age were 1240 g (750 to 2890 g) and 28 weeks (26-35 weeks), respectively. The symptoms took place at a median age of 48 days (9-103 days). All cases initially presented with recurrent apnea and 4 necessary for the assisted fan required with CPAP (2) and mechanical ventilation (2). Serious complications have occurred in 3 infants (2 with a necroting enterocolite and 1 with meningitis) and no deceased. EV has been detected from the relevant swabs (n = 6), csf (n = 2) and nasopharyngeal (n = 2). The viral subtyping revealed an Echovirus 25. The monitoring of all infants exposed by nasopharyngeal swabs was implemented, as well as strict contact precautions and coherence measures.

Conclusions: Premature infants with EV are more subject to serious complications, which can lead to significant morbidity. Thus, early recognition of symptoms, rapid diagnosis and rapid implementation of infection control measures are essential to prevent propagation of subsequent infection.

 

Kallikreins of human tissue in polymorphic adenocarcinoma: a polymerase chain reaction and an immunohistochemical study

The polymorphic adenocarcinoma (PAC) is the second most common tumor of malignant salivary gland of minor salivary glands. The Kallikreins (KLKS) human tissues are a very preserved family of proteases expressed by various tissues and organs. The literature demonstrates a link between klks and the salivary neoplasms of the gland. The purpose of this study was to determine KLK mRNA levels in CAP tissue samples and determine whether the KLK expression is limited to tumor cells. Nineteen CAP cases were examined (1987-2013). The diagnosis has been confirmed, demographic data has been collected and samples of formulated fixed salivary tissues and fixed paraffin. RNA insulation has been achieved, followed by complementary DNA conversion through reverse transcription. By using PCR, the quantitative level of expression of KLKS1-15 has been recorded.

Samples with high and low kLK expression were selected for the coloring of immunohistochemistry. The results revealed a statistically significant increase in KLK average KLK mRNA expression for KLK1, KLK4, KLK10, KLK12 and KLK15 in PAC tissue samples, compared to normal salivary gland fabric (U Mann-Whitney U test, P <0.05). The results of immunohistochemistry have shown that KLKS was present in tumor cells. In particular, all samples demonstrating a relatively higher KLK mRNA expression showed equivalent or increased staining scores compared to low KLK mRNA expression samples. In conclusion, there appears to be an aberrant kallikrein expression in polymorphic adenocarcinoma, suggesting that the possibility of influence on Kallikrein’s waterfall on the development and progression of the tumor. Opportunine identification of etiological agents of enteric infections is necessary to reduce the burden of infantile diarrheal mortality.

The detection methods based on the amplification of the nucleic acid provide a fast and reliable way for the diagnosis of microbes in clinical samples. This study was undertaken to evaluate a conventional conventional polymeric chain reaction of conventional conventional transcription (RT-PCR) conventional (RT-PCR) transcription “developed at the Indian Council for Medical Research.

Successful containment of horizontal enterovirus infection in a neonatal unit in Singapore through diagnosis by polymerase chain reaction (PCR) and direct sequence analysis
Successful containment of horizontal enterovirus infection in a neonatal unit in Singapore through diagnosis by polymerase chain reaction (PCR) and direct sequence analysis

Double Disclosure Probes Improve the sensitivity of detection to acute respiratory syndrome Coronavirus 2 (SARS-COV-2) in a reaction of the reverse transcription polymerase chain (RT-PCR)

Background: Serious Respiratory Syndrome Coronavirus 2 (SARS-COV-2) that emerged in the city of Wuhan, Province of Hubei, China, spread all over the world and threatens human life. The detection of SARS-COV-2 is essential to prevent new epidemics, the retrocedure of the distribution of diseases and the management of patients. Currently, a reaction of the reverse transcription polymerase chain (RT-PCR) is used to detect the virus in clinical laboratories. However, although this test is considered a high specificity, its sensitivity would be as low as 60-70%. Improved sensitivity is therefore urgently required.

Methods: We used the first-handed program and a range recommended by the CDC (N1, N2 and N3) in the United States and NIID (N1 and N2) in Japan. In addition, we have designed double mess probes according to the virus sequence provided by the NIID to develop another test (called the YCH test [N1 and N2]). Using these tests, we made RT-PCR with serial diluted DNA positive controls to evaluate and compare the detection sensitivity of the three tests. In addition, 66 nasopharyngeal swabs were tested to determine diagnostic performance.

Results: The value of the RT-PCR threshold cycle (CT) was relatively small for CDC and YCH analyzes in relation to the NIID test. Serial dilution tests have shown that CDC and YCH analyzes could detect low copy numbers of the positive control of DNA. The starting background fluorescence signal was lower for the YCH test compared to the NIID test. We evaluated the diagnostic performance between single probes (NIID) and double incorrect (YCH) using 66 nasopharyngeal buffers. When the results of the YCH-N2 analysis were used as a reference, each test has detected SARS-COV-2 with 56% positive percentage agreements for NIID-N1, 61% for YCH-N1 and 94% for NIID -N2, and 100% of negative agreements for NIID-N1, YCH-N1 and NIID-N2.

DNA Polymerase Antibody

8C10144 50ug
EUR 368
Description: DNA Polymerase ζ Antibody

RNA Polymerase II antibody

10R-2395 5 mL
EUR 318
Description: Mouse monoclonal RNA Polymerase II antibody

RNA Polymerase II antibody

10R-R116a 100 uL
EUR 548
Description: Mouse monoclonal RNA Polymerase II antibody

DNA Polymerase β Antibody

33357 100ul
EUR 319

DNA Polymerase β Antibody

33357-100ul 100ul
EUR 302.4

DNA Polymerase β Antibody

33357-50ul 50ul
EUR 224.4

DNA Polymerase ζ Antibody

33602 100ul
EUR 319

DNA Polymerase ζ Antibody

33602-100ul 100ul
EUR 302.4

DNA Polymerase ζ Antibody

33602-50ul 50ul
EUR 224.4

DNA Polymerase θ Antibody

33611 100ul
EUR 319

DNA Polymerase θ Antibody

33611-100ul 100ul
EUR 302.4

DNA Polymerase θ Antibody

33611-50ul 50ul
EUR 224.4

DNA Polymerase α Antibody

33665 100ul
EUR 319

DNA Polymerase α Antibody

33665-100ul 100ul
EUR 302.4

DNA Polymerase α Antibody

33665-50ul 50ul
EUR 224.4

DNA Polymerase λ Antibody

34098-100ul 100ul
EUR 302.4

DNA Polymerase λ Antibody

34098-50ul 50ul
EUR 224.4

DNA Polymerase β Antibody

E033357 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E033602 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E033611 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E033665 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase λ Antibody

E034098 100μg/100μl
EUR 255
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E19-3082 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E18-5235-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase ζ Antibody

E11-10144C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase θ Antibody

E11-10173C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase α Antibody

E11-10322C 100μg
EUR 225
Description: Available in various conjugation types.

DNA Polymerase β Antibody

E11-0173C 100μg/100μl
EUR 225
Description: Available in various conjugation types.

RNA polymerase II Antibody

GWB-5B03A0 0.1 mg Ask for price

RNA Polymerase II, Antibody

GWB-3F12B0 0.1 ml Ask for price

DNA Polymerase λ Antibody

8C11836 50ug
EUR 368
Description: DNA Polymerase λ Antibody

DNA Polymerase β Antibody

8C0173 50ug
EUR 368
Description: DNA Polymerase β Antibody

DNA Polymerase θ Antibody

8C10173 50ug
EUR 368
Description: DNA Polymerase θ Antibody

DNA Polymerase α Antibody

8C10322 50ug
EUR 368
Description: DNA Polymerase α Antibody

RNA polymerase II CTD Antibody

GWB-225CEF 0.1 mg Ask for price

DNA Polymerase beta Antibody

35404 100ul
EUR 479

DNA Polymerase beta Antibody

35404-100ul 100ul
EUR 468

DNA Polymerase beta Antibody

E18-0148-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

E18-0148-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

DF6552 200ul
EUR 420

DNA Polymerase beta Antibody

DF6552-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

DF6552-200ul 200ul
EUR 350

DNA Polymerase zeta Antibody

E38PA4807 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase iota Antibody

E38PA5564 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase beta Antibody

AF0148 200ul
EUR 420

DNA Polymerase beta Antibody

AF0148-100ul 100ul
EUR 280

DNA Polymerase beta Antibody

AF0148-200ul 200ul
EUR 350

DNA Polymerase beta Antibody

49605 100ul
EUR 499

DNA Polymerase beta Antibody

49605-100ul 100ul
EUR 399.6

DNA Polymerase beta Antibody

49605-50ul 50ul
EUR 286.8

DNA Polymerase beta antibody

70R-50226 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase beta antibody

DNA Polymerase beta antibody

70R-31161 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase beta antibody

DNA Polymerase zeta antibody

70R-33711 100 ug
EUR 342
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA Polymerase zeta antibody

70R-33727 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase zeta antibody

DNA polymerase beta Antibody

abx232432-100ug 100 ug
EUR 577.2

DNA Polymerase beta Antibody

20-abx008852
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DNA Polymerase beta Antibody

20-abx013060
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DNA Polymerase beta Antibody

ABF0148 100 ug
EUR 525.6

DNA polymerase beta Antibody

GWB-651A32 0.5 ml Ask for price

RNA polymerase beta Antibody

GWB-DBFD55 0.05 ml Ask for price

DNA polymerase beta Antibody

GWB-C8D577 0.25 ml Ask for price

DNA Polymerase beta Antibody

abx013060-100g 100 µg
EUR 237.5

DNA Polymerase beta Antibody

abx013060-10g 10 µg
EUR 43.75

DNA Polymerase beta Antibody

abx013060-200g 200 µg
EUR 312.5

DNA polymerase beta Antibody

abx232432-100g 100 µg
EUR 350

DNA Polymerase Mu (POLM) Antibody

20-abx323923
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DNA Polymerase Mu (POLM) Antibody

abx236614-100ug 100 ug
EUR 661.2

DNA Polymerase Mu (POLM) Antibody

20-abx217851
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DNA Polymerase Mu (POLM) Antibody

20-abx141146
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DNA Polymerase Nu (POLN) Antibody

abx028202-400ul 400 ul
EUR 627.6

DNA Polymerase Nu (POLN) Antibody

abx028202-80l 80 µl
EUR 343.2

DNA Polymerase Mu (POLM) Antibody

abx236614-100g 100 µg
EUR 350

DNA Polymerase Nu (POLN) Antibody

abx028202-400l 400 µl
EUR 518.75

DNA Polymerase Mu (POLM) Antibody

abx323923-100g 100 µg
EUR 250

DNA Polymerase Mu (POLM) Antibody

abx323923-50g 50 µg
EUR 187.5

RNA polymerase II RBP8 Antibody

GWB-80D2DE 0.05 ml Ask for price

DNA Polymerase gamma antibody

22870 100ul
EUR 479

DNA Polymerase gamma antibody

22870-100ul 100ul
EUR 468

DNA Polymerase lamda Antibody

34098 100ul
EUR 319

DNA Polymerase alpha Antibody

DF3082 200ul
EUR 420

DNA Polymerase alpha Antibody

DF3082-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

DF3082-200ul 200ul
EUR 350

DNA Polymerase theta Antibody

DF13563 100ul
EUR 420

DNA Polymerase theta Antibody

DF13563-100ul 100ul
EUR 280

DNA Polymerase theta Antibody

DF13563-200ul 200ul
EUR 350

DNA Polymerase alpha Antibody

AF5235 200ul
EUR 420

DNA Polymerase alpha Antibody

AF5235-100ul 100ul
EUR 280

DNA Polymerase alpha Antibody

AF5235-200ul 200ul
EUR 350

DNA Polymerase alpha antibody

70R-31578 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase alpha antibody

DNA Polymerase theta antibody

70R-33737 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase theta antibody

DNA polymerase gamma antibody

70R-12532 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal DNA polymerase gamma antibody

DNA Polymerase alpha Antibody

20-abx013375
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DNA polymerase delta Antibody

GWB-86F322 0.25 mg Ask for price

DNA polymerase delta Antibody

GWB-7399F1 0.05 ml Ask for price

DNA polymerase delta Antibody

GWB-C04C62 0.1 mg Ask for price

DNA Polymerase alpha Antibody

abx013375-100g 100 µg
EUR 237.5

DNA Polymerase alpha Antibody

abx013375-10g 10 µg
EUR 43.75

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EUR 312.5

DNA Polymerase Eta (POLH) Antibody

20-abx302606
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  • 100 ug
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DNA Polymerase Eta (POLH) Antibody

abx036483-100ug 100 ug
EUR 469.2

DNA Polymerase Eta (POLH) Antibody

abx117166-100ug 100 ug
EUR 560.4

DNA Polymerase Eta (POLH) Antibody

20-abx001515
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DNA polymerase eta Antibody / POLH

R32359 100 ug
EUR 356.15
Description: DNA polymerase eta (POLH), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

DNA Polymerase Eta (POLH) Antibody

abx001515-100l 100 µl
EUR 400

DNA Polymerase Eta (POLH) Antibody

abx001515-20l 20 µl
EUR 175

DNA Polymerase Eta (POLH) Antibody

abx001515-50l 50 µl
EUR 275

DNA Polymerase Eta (POLH) Antibody

abx302606-100g 100 µg
EUR 362.5

DNA Polymerase Eta (POLH) Antibody

abx302606-20g 20 µg
EUR 162.5

DNA Polymerase Eta (POLH) Antibody

abx302606-50g 50 µg
EUR 250

DNA Polymerase Eta (POLH) Antibody

abx117166-100g 100 µg
EUR 450

DNA Polymerase Eta (POLH) Antibody

abx036483-100g 100 µg
EUR 337.5

Anti-DNA Polymerase Antibody

E38A6546 100ug/100ul
EUR 225
Description: Available in various conjugation types.

Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)

TA308753 100 µl Ask for price

DNA Polymerase alpha 1 Antibody

E38PA1906 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase delta 1 Antibody

E38PA1908 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase delta 3 Antibody

E38PA2506 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase gamma 2 Antibody

E38PA4161 100ul
EUR 225
Description: Available in various conjugation types.

DNA Polymerase alpha 1 antibody

70R-50225 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase alpha 1 antibody

DNA Polymerase delta 1 antibody

70R-50227 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase delta 1 antibody

DNA Polymerase delta 3 antibody

70R-50825 100 ul
EUR 242
Description: Purified Polyclonal DNA Polymerase delta 3 antibody

DNA Polymerase delta 3 Antibody

20-abx008311
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  • 100 ul
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DNA Polymerase delta 1 Antibody

20-abx008851
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  • 100 ul
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DNA Polymerase alpha 1 Antibody

20-abx008853
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  • 100 ul
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RNA polymerase II RPB11 Antibody

GWB-81535B 0.05 ml Ask for price

RNA polymerase sigma 70 Antibody

GWB-68CBEA 0.05 ml Ask for price

DNA Polymerase lambda antibody

70R-32186 100 ug
EUR 294
Description: Rabbit polyclonal DNA Polymerase lambda antibody

anti- DNA polymerase beta antibody

FNab02432 100µg
EUR 606.3
Description: Antibody raised against DNA polymerase beta

Anti-DNA Polymerase δ Antibody

13910 100ug
EUR 503

Anti-RNA Polymerase II Antibody

70101 200ug
EUR 441

DNA Polymerase Iota (POLI) Antibody

20-abx323859
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  • 100 ug
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DNA Polymerase Iota (POLI) Antibody

20-abx333843
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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DNA Polymerase Iota (POLI) Antibody

abx036752-100ug 100 ug
EUR 469.2

Conclusion: Double-discarded probes decreased the bottom fluorescence and improved the RT-PCR detection sensitivity for SARS-COV-2.