Cutaneous leishmaniasis: a pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis
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Cutaneous leishmaniasis: a pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis
BACKGROUND: Traditional methods for the diagnosis of leishmaniasis give low sensitivity, which limits its effectiveness in lesions with low parasite burden.
Methods: Retrospective Pathological Study of 360 Cases of Cutaneous Lishmaniasis and Analysis of the Different Diagnostic Methods used.
Results: In 93% of lesions, histopathology showed a dense and diffuse inflammatory infiltrate, consisting of lymphocytes, histiocytes and plasma cells, which occupied the superficial and medium dermis and variedly to the deep dermis and the Superficial subcopper (standard pattern). The remaining cases showed atypical characteristics, such as perivascular, interstitial or perifollicular inflammatory patterns, folliculitis or guests. The granulomas were identified in 84% of biopsies, most of them like small non-necroting non-necroting histiocyte aggregates. The amastigotes were visualized by a histopathological examination of routine in 36% of biopsies. Immunohistochemistry stained 17/26 lesions (65%) negative by conventional spots. The PCR provided the correct diagnosis in 218 cases (58% of the negative series) for Laishmania by other techniques.
Conclusions: negative biopsies for Leishmania by traditional diagnostic methods that show that the histopathological standard model or the atypical characteristics of patients with clinical suspicion of cutaneous leishmaniasis in endemic areas should be studied by immunohistochemistry and / or PCR for Leishmania to reach the definitive diagnosis. . This article is protected by copyright. All rights reserved. In Japan, Buruli ulcer affairs are often advanced, requiring surgical treatment. However, extended debridement is often difficult due to cosmetic and functional sequelae. In addition, the lesions are complicated and composed of erratic erythemum, necrotic ulcer and erythematous skin lesions caused by a paradoxical reaction, which also makes it difficult to achieve adequate debridement.
Raman vs spectroscopy Quantitative reaction of the Polymerase chain at the Early Stage Huanglongbing Diagnostics
Raman (RS) spectroscopy is an emerging analytical technique that can be used to develop and deploy precision agriculture. RS allows a diagnosis of confirmation of biotic and abiotic stresses on plants. More specifically, RS can be used for Huanglongbing (HLB) diagnostics on orange and grapefruit trees, as well as detecting and identifying various fungal and viral diseases. The questions that remain to be solved are how fast can detect and identify the disease and if RS is more sensitive than the QPCR, the “Golden Standard” in pathogenic diagnostics? Using RS and HLB as a case study, we have monitored healthy citrus (QPCR-negative) and compared their spectra to spectra collected from orange trees and healthy grapefruit cultivated in a greenhouse with access to restricted insects and confirmed as HLB for free by QPCR.
Our result indicated that RS was capable of early prediction of HLB and that almost all field-negative plants were infected with the disease. Using advanced multivariate statistical analysis, we have also shown that QPCR-negative plants showed specific HLB spectral characteristics that can be distinguished from unrelated nutrition deficit characteristics. These results demonstrate that RS is capable of much more sensitive diagnostics of HLB compared to QPCR.
We have acquired common fluid samples (JFSS) by preoperative aspiration of suspected patients to have a failed PJI and arthroplasty; Patients with preoperative JFS volumes less than 5 ml have been enrolled. RNA polymerase chain reactions (PCR) and bacterial culture have been performed and diagnostic efficiency has been compared between the two methods.Self the criteria of the established musculoskeletal infection society (MSIS) , 21 of the 33 patients included were diagnosed with PJI.
Cutaneous leishmaniasis: a pathological study of 360 cases with special emphasis on the contribution of immunohistochemistry and polymerase chain reaction to diagnosis
Real-timely improved quantitative polymerase reaction technology for helicobacter pylori detection in stomach tissues and application value in clinical precision tests
Background: Helicobacter pylori (H. pylori) infection is a serious threat of human health. The empirical treatment paradigm H. pylori guided by traditional test technologies has led to antibiotic resistance. Here we have improved the QPCR method to provide technical support for H. pylori precision diagnosis and treatment.
Methods: Two pairs of primers and probes targeting the GLMM gene have been designed to detect H. pylori and a QPCR multiplex method has been put in place for virulence factor detection. Then, a rapid test of urease (RUT), the culture and the QPCR were carried out on 141 specimens collected from the China Xinqiao Hospital in 2017 to evaluate the detection capacity of the QPCR. Finally, the infectious amount of H. pylori and virulence genes were detected by QPCR.
Results: 1. The improved QPCR method that used two primer pairs had a higher detection rate (100%) and better accuracy (p = 0.000), compared to QPCR using a pair of primers. It has also had a better consistency with bacterial culture than with the rut (kappa = 0.440, p <0.001). 2. The infectious amount H. pylori was significantly positively to gastritis in the corpus (p = 0.003) and gastric erosion (p = 0.043). Infectious infectious amount H. pylori of gastric precancerous patients was significantly lower than that of pylorior-positive patients (p <0.05), and the infectious amount H. Pylori-Vaca S1 + was significantly higher than H. pylori- Vaca S1 – (p <0.05). 3. The Vaca S1 frequency was significantly higher than that of Vaca M1 / Caga + / Baba2 + in chronic superficial gastritis (P = 0.000), peptic ulcer (p = 0.037) and gastric erosion (p = 0.009). The frequency H. pylori-Vaca + / Caga + / Baba2 + showed a significant positive correlation (p <0.05).
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Rat DNA polymerase delta catalytic subunit (POLD1)
Description: Quantitative sandwich ELISA for measuring Rat DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Human DNA Polymerase Delta 1, Catalytic Subunit (POLD1) ELISA Kit
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse DNA polymerase delta catalytic subunit (POLD1)
Description: Quantitative sandwich ELISA for measuring Mouse DNA polymerase delta catalytic subunit (POLD1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Human DNA polymerase epsilon catalytic subunit A (POLE) ELISA Kit
Conclusions: The infectious amount H. pylori and the presence of Virulence H. pylori factors have shown complex correlations with the occurrence and development of gastric diseases. The improved QPCR with good detection performance can be used for the quantitative detection of H. pylori and tests for Vaca S1 virulence genes, Vaca M1, Caga and Baba2 simultaneously. These results will provide valuable information for the diagnosis and treatment of diseases.
