Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
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Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
Objective: sexually transmitted infections due to Neisseria gonorrhoeae (NG) and / or Chlamydia trachomatis (CT) remains a major public health problem. Although the literature describes the epidemiology is based on a population of CT / NG, does not appear to contain a reference point for the statistical analysis of the level of positive specimens by nucleic acid testing (NAT) by the polymerase chain reaction (PCR) which will be collected by the following laboratory best laboratories and practice rules.
For facilities that NG and CT diagnostics by real-time PCR, an understanding of the level of expected positive gonorrhea and chlamydia specimens will help to monitor the assay for quality assurance. Therefore, on behalf of Michael J. Crescenz Veterans Affairs Medical Center (VAMC), we present a new quality assurance study on CT / NG specimens positive rate conducted by the NAT with PCR.
Methods: The quality assurance / improvement of quarterly data from April 1, 2012 to September 30, 2019 are reviewed to get a good volume PCR test for CT / NG and the number of positive test results in the VAMC to compose and perform statistical analysis. Tests have been conducted using the Abbott m2000 RealTime system (Abbott Park, IL).
Results: A total of 22 709 PCR test for CT / NG has been done on veteran population; these, 502 tested positive for NG and 744 positive for CT. quarterly percentage rate ranging between 1.67% to 5.30% for the CT and from 1.00% to 3.25% for NG, with an average rate of 3.35% and 2.22% for CT and NG, respectively -masing.
Conclusion: The establishment of the expected level of positive specimens from CT / NG with NAT with PCR at VAMC is a significant new reference point in quality assurance (QA) literature and provide a benchmark which helps greatly in QA for microbiological / molecular lab.
Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
Fast and reliable detection of carbapenemase gene in various Gram negative using real-time polymerase chain reaction-based platform commercially available fluorescence new
The Revogene® Carba C assay is a real-time polymerase chain reaction-based assay that runs on Revogene microfluidic platform. Recently designed to detect the gene encoding the major carbapenemases 5 (NDM, VIM, IMP, KPC, and Oxa-48) of various Gram-negative. A total of 145 clinical strains of Gram-negative (96 carbapenemase producers and non-producers carbapenemase 49) were tested.
The overall sensitivity and specificity of 100%. All strains co-producing double carbapenemases correctly detected. All manufacturers of non-carbapenemase and nontargeted carbapenemase producers gave negative results. Sample preparation is easy to handle, take about 5 to 10 minutes per isolates, with a running time of about 70 minutes.
This test is an easy-performing, fast reliable tool to detect the most common carbapenemases, with excellent sensitivity and specificity in spite of the bacterial host. Given the user friendliness, simplicity, and a short time to result, Revogene® test Carba C suitable for the microbiology lab. Sapoviruses increasingly recognized as pathogens associated with gastroenteritis in humans. human sapoviruses currently assigned to the 18 genotypes (GI.1-7, GII.1-8, GIV.1, and GV.1-2) based on the order of the main structural protein coding region.
Description: Quantitative competitive ELISA kit for measuring Human Vitamin B12, VB12 in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitative competitive ELISA kit for measuring Human Vitamin B12, VB12 in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Vitamin B12 (VB12) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Vitamin B12 (VB12) in samples from serum, plasma, tissue homogenates or other biological fluids.
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Description: Quantitative competitive ELISA kit for measuring Rat Vitamin B12, VB12 in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Goat vitamin B12 (VB12) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Goat vitamin B12 (VB12) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
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Description: Quantitative competitive ELISA kit for measuring Mouse Vitamin B12, VB12 in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
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Description: Quantitative competitive ELISA kit for measuring Bovine Vitamin B12, VB12 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitative sandwich ELISA for measuring Rat Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Rat Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Rat Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Mouse Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Bovine Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Bovine Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Quantitative sandwich ELISA for measuring Bovine Vitamin B12 (VB12) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Bovine Vitamin B12,VB12 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Human Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production. Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Competitive Immunoassay;Sensitivity: < 1.498 pg/mL
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Description: A competitive ELISA for quantitative measurement of Canine Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Description: A competitive ELISA for quantitative measurement of Porcine Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Description of target: It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production. Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.;Species reactivity: All;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.051 ng/mL
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Description: A competitive ELISA for quantitative measurement of Guinea pig Vitamin B12 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Enzyme-linked immunosorbent assay kit for quantification of General Vitamin B12 in samples from serum, plasma, tissue homogenates and other biological fluids.
In this study, we evaluated 11 polymerase chain reaction (PCR) assay using published and newly designed / modified primary and indicate that the four PCR with a combination of different primary amplified all genotypes sapovirus tested humans using DNA either synthetic or cDNA prepared from sapovirus human specimen stool-positive. This test can be used as a screening test extensively enhanced reactive or as a tool for the molecular characterization of human sapoviruses.
