Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
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Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
Objective: sexually transmitted infections due to Neisseria gonorrhoeae (NG) and / or Chlamydia trachomatis (CT) remains a major public health problem. Although the literature describes the epidemiology is based on a population of CT / NG, does not appear to contain a reference point for the statistical analysis of the level of positive specimens by nucleic acid testing (NAT) by the polymerase chain reaction (PCR) which will be collected by the following laboratory best laboratories and practice rules.
For facilities that NG and CT diagnostics by real-time PCR, an understanding of the level of expected positive gonorrhea and chlamydia specimens will help to monitor the assay for quality assurance. Therefore, on behalf of Michael J. Crescenz Veterans Affairs Medical Center (VAMC), we present a new quality assurance study on CT / NG specimens positive rate conducted by the NAT with PCR.
Methods: The quality assurance / improvement of quarterly data from April 1, 2012 to September 30, 2019 are reviewed to get a good volume PCR test for CT / NG and the number of positive test results in the VAMC to compose and perform statistical analysis. Tests have been conducted using the Abbott m2000 RealTime system (Abbott Park, IL).
Results: A total of 22 709 PCR test for CT / NG has been done on veteran population; these, 502 tested positive for NG and 744 positive for CT. quarterly percentage rate ranging between 1.67% to 5.30% for the CT and from 1.00% to 3.25% for NG, with an average rate of 3.35% and 2.22% for CT and NG, respectively -masing.
Conclusion: The establishment of the expected level of positive specimens from CT / NG with NAT with PCR at VAMC is a significant new reference point in quality assurance (QA) literature and provide a benchmark which helps greatly in QA for microbiological / molecular lab.
Gonorrhea and Chlamydia Specimen Positivity Rate by Polymerase Chain Reaction at a Regional Veteran Affairs Medical Center
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